Origins of Atlantic Salmon (Salmo salar) Determined Using a Hybridization Assay of Mitochondrial DNA on a Microfluidic Biochip

Abstract

A hybridization assay based on the microfluidic biochip was developed to identify the origin of the Atlantic salmon species. Among the 215 single nucleotide polymorphic (SNP) sites found in the mtDNA of Salmo salar, we located five sites in devising our assay method. We found two sites that worked, while the others generated either insufficient signals or specificity. We have successfully identified the North American origin of the three samples, as confirmed by Sanger sequencing.